Comparative studies on the influence of extraction methods on amino acid and functional properties of black benniseed (Sesamum radiatum) and false sesame (Ceratotheca sesamoides) protein concentrates

Abstract

Usage of plant proteins for development and formulation of functional food products has taken centre stage. In this study, functional parameters and amino acid profile of protein concentrates extracted from false sesame and black benniseed were appraised.  The extraction was carried using isoelectric precipitation and ammonium sulphate precipitation methods. The protein yield was higher for the Isoelectric precipitation method (28.25-33.99%) than ammonium sulphate (13.36-30.72%). In the same vein, isoelectric precipitated concentrates had higher protein content (76.17 and 68.60%) than ammonium sulphate concentrate (60.02 and 65.8%). However, for functional properties ammonium sulphate concentrate gave improved functional parameter results in terms of water absorption capacity (208.24-225.62%), oil absorption capacity (175.60-218.95%), and swelling capacity (184.83-219.79%). The total essential, non-essential, hydrophobic, and hydrophilic amino acids content was higher in concentrates gotten from ammonium sulphate precipitation method than the concentrates gotten from isoelectric precipitation method. Hence, our findings suggest that ammonium sulphate method may be the most applicable method for promoting enhanced functionality and utilization of false sesame and black benniseed protein concentrates.Usage of plant proteins for development and formulation of functional food products has taken centre stage. In this study, functional parameters and amino acid profile of protein concentrates extracted from false sesame and black benniseed were appraised.  The extraction was carried using isoelectric precipitation and ammonium sulphate precipitation methods. The protein yield was higher for the Isoelectric precipitation method (28.25-33.99%) than ammonium sulphate (13.36-30.72%). In the same vein, isoelectric precipitated concentrates had higher protein content (76.17 and 68.60%) than ammonium sulphate concentrate (60.02 and 65.8%). However, for functional properties ammonium sulphate concentrate gave improved functional parameter results in terms of water absorption capacity (208.24-225.62%), oil absorption capacity (175.60-218.95%), and swelling capacity (184.83-219.79%). The total essential, non-essential, hydrophobic, and hydrophilic amino acids content was higher in concentrates gotten from ammonium sulphate precipitation method than the concentrates gotten from isoelectric precipitation method. Hence, our findings suggest that ammonium sulphate method may be the most applicable method for promoting enhanced functionality and utilization of false sesame and black benniseed protein concentrates.