Attenuation of Rutin on Butachlor-Induced Hepatotoxicity in Rats

Ebenezer Tunde Olayinka(1), Samuel Abiodun Kehinde(2), Abosede Temitope Olajide(3),


(1) Biochemistry Program, Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Nigeria
(2) Department of Environmental Health Sciences, Ajayi Crowther University, Oyo, Nigeria.
(3) Biochemistry Program, Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University, Oyo, Nigeria
Corresponding Author

Abstract


Butachlor, a chloroacetanilide herbicide, is used globally as pre-emergence control of unwanted weeds and concerns about its potential adverse effects and toxicity have risen. Rutin is a natural flavonoid with hepatoprotective potentials. The current study aims to investigate the hepatoprotective effect of Rutin on Butachlor-induced hepatotoxicity in rats. Twenty-four male wistar rats weighing between 260 g – 280g were randomized into four (4) groups of six animals each. Group A (control) received only distilled water, group B (Butachlor) received 100 mg/kg body weight (B.W.) Butachlor, and group C (Butachlor + Rutin) received 100 mg/kg B.W. Butachlor and 100 mg/kg Rutin while group D (Rutin) received 100 mg/kg B.W. Rutin. Administration lasted for 14 days and the rats were sacrificed, the liver excised and further processed for biochemical analysis. Administration of Butachlor resulted in hepatotoxicity that was characterized by a significant increase in plasma ALT, AST and ALP levels relative to the control group (p<0.05). Also, a significant increase (p<0.05) in the level of MDA and NO of Butachlor-treated rats were observed when compared with the control group. Furthermore, altered antioxidant status was observed as activities of enzymatic antioxidants (SOD, CAT, and GST) and concentration of non-enzymatic antioxidants (GSH and AA) were down-regulated. However, co-administration of Rutin with Butachlor significantly attenuated all the alterations in the markers of liver injury and oxidative stress relative to the Butachlor-treated group. Data obtained from this study showed that Rutin offered a protective effect against Butachlor-induced hepatotoxicity in rats.


Keywords


Butachlor; Rutin; Herbicide; Hepatotoxicity; Anti-oxidants; Oxidative stress

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